Journal: STAR Protocols
Article Title: Protocol for generation of human gallbladder epithelia cells and their derived hepatocytes using a chemically defined approach
doi: 10.1016/j.xpro.2025.104302
Figure Lengend Snippet: Production and identification of human gallbladder epithelial cells (hGBECs) (A) Schematic diagram for the procedure of production and identification of hGBECs. (B) q-RT-PCR for expression of KRT19, EPCAM, LGR5, SOX9, HNF4A and ALB in each passage of hGBECs. Fold chang is calculated by 2 -ΔΔCt , relative to GAPDH (n= 3). Data are represented as mean ± SD. (C) Immunostaining for HNF4A, SOX9 (red), TROP2 and EPCAM (green) of hGBECs. Cell nuclei were stained by DAPI (blue). Scale bar = 100 μm.
Article Snippet: Anti-human SOX9 rabbit primary antibody (1:200 IF) , Merck , Cat#HPA001758; RRID: AB_1080067.
Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Immunostaining, Staining